논문검색
Author Su Mi Bae, Yong Wook Kim, Joon Mo Lee, Sung Eun Namkoong, Sei Jun Han, Jong Ki Kim, Chang Hee Lee, Heung Jae Chun, Hyun Sun Jin, Woong Shick Ahn
Place of duty 1Catholic Research Institutes of Medical Science, 2Department of Obstetrics and Gynecology, The Catholic University of Korea College of Medicine, Seoul, 3Department of Obstetrics and Gynecology, The Catholic University of Korea College of Medicine, Daegu, 4Department of Chemistry, Kangwon University, Gangwondo, Korea.
Title Photodynamic Effects of Radachlorin on Cervical Cancer Cells
Publicationinfo Cancer Research and Treatment 2004 Dec; 036(06): 389-394.
Key_word Radachlorin,Photodynamic therapy (PDT),Cervical cancer,TC-1 cell
Full-Text
Abstract Purpose: Photodynamic therapy (PDT) is a novel treatment modality, which produces local tissue necrosis with laser light following the prior administration of a photosensitizing agent. Radachlorin has recently been shown to be a promising PDT sensitizer. In order to elucidate the antitumor effects of PDT using Radachlorin on cervical cancer, growth inhibition studies on a HPV-associated tumor cell line, TC-1 cells in vitro and animals with an established TC-1 tumor in vivo were determined. Materials and methods: TC-1 tumor cells were exposed to various concentrations of Radachlorin and PDT, with irradiation of 12.5 or 25 J/cm2 at an irradiance of 20 mW/cm2 using a Won-PDT D662 laser at 662 nm in vitro. C57BL/6 mice with TC-1 tumor were injected with Radachlorin via different routes and treated with PDT in vivo. A growth suppression study was then used to evaluate the effects at various time points after PDT. Results: The results showed that irradiation of TC-1 tumor cells in the presence of Radachlorin induced significant cell growth inhibition. Animals with established TC-1 tumors exhibited significantly smaller tumor sizes over time when treated with Radachlorin and irradiation. Conclusion: PDT after the application of Radachlorin appears to be effective against TC-1 tumors both in vitro and in vivo. (Cancer Research and Treatment 2004;36: 389-394)